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Limited long-term impact of insect venom immunotherapy on the micro-RNA landscape in whole blood

Karpinski P1*, Kahraman M2*, Ludwig N3, Skiba P1, Kosinska M4, Rosiek-Biegus M4, Królewicz E4, Panaszek B4, Nittner-Marszalska M4, Blin N1, Keller A2, Meese E3, Malgorzata Sasiadek M1

1Department of Genetics; Wroclaw Medical University, Wroclaw, Poland
2Chair for Clinical Bioinformatics, Saarland University, Saarbrücken, Germany
3Institute of Human Genetics, Saarland University, Homburg, Germany
4Department and Clinic of Internal Medicine and Allergology; Wroclaw Medical University, Poland

J Investig Allergol Clin Immunol 2019; Vol. 29(3)
doi: 10.18176/jiaci.0303

Objective: To perform a genome-wide characterization of changes of microRNA (miRNA) expression in the course of VIT (venom immunotherapy).
Methods: miRNA was isolated form in the whole-blood of 13 allergic patients and 14 controls, who showed no allergic reaction upon stings by honeybees and wasps. We analyzed 2549 different miRNAs from whole blood of these patients prior to VIT and 12 months after the start of the VIT. Differential expression results obtained on microarray platform were confirmed by quantitative real -time PCR (qRT-PCR). Out of the 13 patients, eight were confirmed to show a negative allergic reaction upon VIT thus indicating a successful VIT.
Results: By comparing time points prior and 12 month after ultra-rush venom immunotherapy (VIT), correlation and principal component analysis both indicate a limited effect of the VIT on the overall miRNA expression pattern. Volcano blot analysis based on raw p-values revealed few deregulated miRNAs with the majority of them being increasingly expressed after VIT as compared to prior VIT. Using the 50 most altered miRNAs, there was no clear clustering according to the time points i.e. the time prior and the time after VIT.
Conclusions: Our results indicate an overall low effect of VIT on the miRNA expression pattern in whole blood. 

Key words: miRNA, Whole genome, Blood, Expression, Venom immunotherapy