Promoter genotyping and mRNA expression analysis of PTGDR gene in allergy
San Segundo-Val I1, García-Sánchez A2,3, Sanz C2,4, Cornejo-García JA5, Isidoro-García M1,2,6, Dávila I2,3,7
1Department of Clinical Biochemistry, University Hospital of Salamanca, Spain.
2Institute for Biomedical Research of Salamanca, Spain.
3Department of Biomedical Sciences and Diagnostics, University of Salamanca, Spain.
4Department of Microbiology and Genetics, University of Salamanca, Spain.
5Research Laboratory, IBIMA, Regional University Hospital of Malaga, UMA, Malaga, Spain.
6Department of Medicine, University of Salamanca, Spain.
7Department of Allergy, University Hospital of Salamanca, Spain
J Investig Allergol Clin Immunol 2020; Vol. 30(2)
Background: Prostaglandin D2 receptors are acquiring a relevant role as potential therapeutic targets in allergy. PTGDR has been described, as a candidate gene in allergic disease however there is a lack of functional studies on this gene.
Objective: the aim of this case-control study was to interrogate the potential role of PTGDR in allergy.
Methods: 195 allergic patients and 112 healthy controls were included. The PTGDR promoter polymorphic positions -1289G>A, -1122T>C, -881C>T, -834C>T, -613C>T, -549T>C, -441C>T, -197T>C and -95G>T were amplified by polymerase chain reaction and sequenced. PTGDR expression levels were analyzed by q-PCR and normalized to GAPDH and TBP mRNA levels. All procedures were performed following MIQE guidelines.
Results: PTGDR expression levels were significantly higher in allergic patients than in controls (P<0.001). ROC analysis for PTGDR expression showed a sensitivity of 81.4% compared to 67% for IgE levels. In addition, differences in the genotypic distribution of -1289G>A and -1122T>C polymorphisms were found in allergic patients (P = 0.009).
Conclusions: The results indicate that PTGDR overexpression is associated with allergy. In addition, polymorphisms -1289G>A and -1122T>C contribute to explain some of the observed expression variation. PTGDR expression could have a potential role as biomarker and pharmacogenetic factor in Allergy.
Key words: Allergy; Asthma; mRNA Expression; Polymorphisms; PTGDR; Rhinitis