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Salsola kali cross-reacts extensively with Salsola imbricata

Al-Ahmad M1, Rodriguez-Bouza T2, Fakim N2, Pineda F3
1Microbiology Department, Faculty of Medicine, Kuwait University
2Al-Rashed Allergy Center, Ministry of Health, Kuwait
3Diater Laboratories, Madrid, Spain

J Investig Allergol Clin Immunol 2018; Vol. 28(1)
doi: 10.18176/jiaci.0204

Background: There are no studies on the cross-reactivity between Salsola kali and Salsola imbricata pollens. The main goal is to compare the degree of the cross-reactivity between Salsola kali and Salsola imbricata, and secondly to compare the various common allergen components between Salsola kali and Salsola imbricata.
Methods: Serum from rhinitis patients with or without asthma living in Kuwait presenting with a positive skin test to Salsola kali were obtained. SDS PAGE/IgE Western blot, ELISA inhibition assay was done with patients from Kuwait.
Results: 37 patients from Kuwait were recluted. The most frequent IgE reactive proteins against Salsola imbricata were around 12, 15, 18, 37 and 50+55 kDa. 2D electrophoresis displayed a correlation between Salsola kali and Salsola imbricata at 40, 60, 75 KDa with similar isoelectric points. ELISA inhibition presented with an Ag50 value of 1.7 μg/mL for Salsola kali and 500.5 μg/mL for Salsola imbricata when the solid phase was Salsola kali, and an Ag50 value of 1.4 μg/mL for Salsola kali and 3.0 μg/mL for Salsola imbricata when solid phase was Salsola imbricata.
Conclusions: Salsola kali and Salsola imbricata present strong cross-reactivity as observed in ELISA inhibition and this might be clinically relevant for the efficacy of allergen specific immunotherapy. We described, for the first time, the allergenic profile for Salsola imbricata and possible common allergenic proteins for Salsola kali and Salsola imbricata.

Key words: Allergens and epitopes, IgE, Immunoblotting, Immunotherapy, Rhinitis, Salsola.