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VEGF -2549 -2567 del18 polymorphism and irreversible bronchoconstriction in asthmatics

Gomulka K1, Liebhart J1, Jaskula E2, Lange A2, Medrala W1

1Department of Internal Medicine and Allergology, Wroclaw Medical University, Wroclaw, Poland.
2Laboratory of Clinical Immunology, L. Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Science and Lower Silesian Cell Transplant Centre with the National Bank of Bone Marrow Donors Wroclaw, Poland

J Investig Allergol Clin Immunol 2019; Vol. 29(6)
doi: 10.18176/jiaci.0369

Background: While the importance of vascular endothelial growth factor (VEGF) for the pathogenesis of several diseases (e.g. neoplasms) has been proven, the corresponding data for asthma, especially those relating to the potential associations between VEGF genetic variants and airway remodeling are relatively scarce.
Objectives: This study was purposed to evaluate the possible connection between a genetic factor which is polymorphism del/ins in the VEGF-promoter region and airway remodelling potential in asthmatics with and without irreversible bronchoconstriction.
Materials and methods: In our study participated 82 patients with asthma (therein 42 patients with irreversible bronchoconstriction) and group of 40 controls. DNA was isolated from peripheral blood leukocytes. The polymerase chain reaction method (PCR) was used for typing of the VEGF (18-bp deletion/insertion) gene polymorphism at -2549 -2567 loci. Other factors (i.e. smoking, disease duration) also were taken under consideration.
Results: The del/del genotype was found in 74.39% patients with asthma (p=0.031; OR=2.38), 80.95% patients with irreversible bronchoconstriction (p=0.012; OR=3.48) and 67.5% patients with reversible bronchoconstriction (p=0.251; OR=1.70). Proportion of tobacco smokers to nonsmokers was higher (p=0.032) and disease duration was longer (p=0.041) in patients with irreversible bronchoconstriction compared to those with reversible bronchoconstriction.
Conclusions: Our results showed that the presence of del18 genotype at -2549 -2567 position in the promoter of VEGF gene, along with disease duration and other factors like cigarette smoking, associate with the risk of irreversible bronchoconstriction in asthmatics.

Key words: Vascular Endothelial Growth Factor (VEGF), Gene Polymorphisms, Asthma, Airway Remodeling, Irreversible Bronchoconstriction