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Original Article |
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Identification of New Potential
Allergens From Nile Perch (Lates niloticus) and
Cod (Gadus morhua) |
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JM Tomm,1 T van Do,2
C Jende,1 JC Simon,3 R Treudler,3
M von Bergen,1,4 M Averbeck3 |
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1Department of Proteomics,
Helmholtz-Centre for Environmental Research, Leipzig,
Germany
2Section for Clinical Allergology, Laboratory
of Clinical Biochemistry, Haukeland University Hospital,
Bergen, Norway
3Department of Dermatology, Venereology and
Allergology, University of Leipzig, Leipzig, Germany
4Department of Metabolomics, Helmholtz-Centre
for Environmental Research, Leipzig, Germany |
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J Investig Allergol Clin Immunol
2013; Vol. 23(3): 159-167 |
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Abstract |
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Background:
Globalization of the
food industry has
led to widespread
exposure to new
nondomestic fish
species; therefore,
identification of
potential allergens
is necessary in
order to diagnose
allergic reactions.
Objective:
Contact with a
patient who was
allergic to Nile
perch (Lates
niloticus)
prompted us to
investigate the
immunoglobulin (Ig)
E–reactive proteins
that could be
allergens of this
species.
Methods: 2D
gel electrophoresis
was used to separate
the muscle proteins
of L niloticus
and Gadus morhua.
Immunoblotting was
performed with sera
from 12 patients
with a history of
immediate-type
allergic reaction to
fish and from atopic
and nonatopic
controls. IgE-reactive
proteins were
detected and
identified using
mass spectrometry.
Results: The
index patient had
low levels of IgE
binding to
parvalbumins.
However, 8 putative
allergens other than
parvalbumin from
L niloticus and
5 from G morhua
were identified.
Further
investigation
revealed
cross-sensitivity to
enolase 3 from L
niloticus in 7
of the 12
fish-allergic
individuals (58%),
whereas 11 of the 12
patients (92%) were
sensitized to
enolase 3 from G
morhua. However,
atopic control
patients were also
sensitized to
enolase 3 from L
niloticus and
G morhua.
Conclusion:
Identification of
species-specific
allergens and
individual
sensitization could
help us to improve
avoidance
strategies.
Key words: 2D
gel electrophoresis.
Gadus morhua.
Lates niloticus.
Fish allergens. IgE.
Western blot. |
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