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Original Article


Intestinal Lamina Propria TcRγδ+ Lymphocytes Selectively Express IL-10 and IL-17


KE Asigbetse, PA Eigenmann, and CP Frossard

Inflammation and Allergy Research Group, Department of Paediatrics and Department of Internal Medicine, Geneva University Hospitals and University of Geneva, Geneva, Switzerland

J Investig Allergol Clin Immunol 2010; Vol. 20(5): 391-401



Background: The characteristics and roles of gut lymphocytes have been only partly elucidated, in particular with regard to activation patterns.

Objectives: To characterize lymphocytes from various parts of the gut and examine their activation pattern as a network.

Methods: Lymphocytes were isolated from the epithelium, the lamina propria, Peyer's patches, mesenteric lymph nodes, the spleen, and peripheral blood of naïve mice. They were then characterized for T cell phenotype, T cell receptors (TcRs), activation markers, and cytokine production.

Results: The results showed a gradient of cells with an increasing proportion of TcRγδ+, CD8αα+ cells towards the gut lumen, with the highest number found in intraepithelial lymphocytes. These cells, together with lamina propria lymphocytes (LPLs) were also characterized by a memory-like phenotype (CD25– CD45RBlow and CD44high) and CD69 expression. CD8+ TcRγδ+ LPLs produced IL-10 and IL-17, while TcRαß+ LPLs were FoxP3 positive.

Conclusions: Gut lymphocytes express various receptors and cytokines according to their location. These specific features suggest a differential function for gut lymphocytes depending on their location.

Key words: TcRγδ+ lymphocytes. IL-17. IL-10.