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Original Article


Lack of Association Between Aspirin-Triggered 15-Hydroxyeicosatetraenoic Acid Release and Mast Cell/Eosinophil Activation in Nasal Polyps From Aspirin-Sensitive Patients


A Lewandowska-Polak,1 M Jędrzejczak-Czechowicz,1 JS Makowska,1 M Jarzębska,1 A Jankowski,2 ML Kowalski1

1Department of Immunology, Rheumatology and Allergy, Chair of Clinical Immunology and Microbiology, Medical University of Łódź, Łódź, Poland
2ENT Department, Medical University of Łódź, Łódź, Poland

J Investig Allergol Clin Immunol 2011; Vol. 21(7): 507-513



Background: The mechanism of aspirin sensitivity in patients with asthma and rhinosinusitis has been attributed to arachidonic acid metabolism abnormalities.

Objective: We aimed to test whether aspirin-triggered generation of 15-hydroxyeicosatetraenoic acid (15-HETE) in nasal polyp dispersed cells (NPDCs) from aspirin-sensitive patients is associated with activation of inflammatory cells.

Methods: Polyps were obtained from 11 aspirin-sensitive and 19 aspirin-tolerant patients with chronic rhinosinusitis. NPDCs were stimulated by aspirin or calcium ionophore. Levels of 15-HETE, leukotriene (LT) C4, eosinophil cationic protein (ECP), and tryptase were measured in NPDC supernatant.

Results: NPDCs from aspirin-sensitive patients contained more eosinophils (14% vs 9%, P<.05) and released 2.4-fold more ECP (P<.01) at baseline. Stimulation with aspirin (200 μM) resulted in a signifi cant increase in 15-HETE generation only in tissue from aspirin-sensitive patients (mean increase, 82%) but did not induce any increase in the release of LTC4, ECP, or tryptase. Preincubation with calcium ionophore
resulted in significantly enhanced generation of 15-HETE, ECP, tryptase, and LTC4 in patients from both groups. Incubation of NPDCs with misoprostol inhibited aspirin-induced 15-HETE generation in aspirin-sensitive patients and calcium ionophore–induced 15-HETE, ECP, and tryptase release in both aspirin-sensitive and aspirin-tolerant patients.

Conclusion: Our study demonstrated that aspirin-induced 15-HETE generation in nasal polyps from aspirin-sensitive patients is not associated with activation of mast cells and eosinophils. Misoprostol has a potent inhibitory effect on the activation of cells derived from the site of nasal mucosal inflammation, regardless of sensitivity to aspirin.

Key words: Aspirin sensitivity. Misoprostol. Nasal polyps. 15-HETE