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SL Porcel Carreño,1 F
Pineda de la Losa,2 EM Frontera Carrión3
AB Sánchez González,4 E Rodríguez Martín,1
S Jiménez Timón,1 M Alvarado Arenas,1
B de la Hoz Caballer,5 MC Diéguez Pastor,5
FJ Hernández Arbeiza1 |
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1Sección de Alergología,
Complejo Hospitalario de Cáceres, Cáceres, Spain
2R+D+I Department, Diater laboratorios, SA,
Madrid, Spain
3Departamento de Parasitología, Facultad de
Veterinaria, Universidad de Extremadura, Cáceres, Spain
4Centro de Salud de Alcántara, Área de Salud
de Cáceres, Alcántara, Spain
5Servicio de Alergología, Hospital Ramón y
Cajal, Madrid, Spain |
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Abstract |
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Background:
Our group previously
found that up to 7%
of amateur anglers
in Caceres, Spain
may be allergic to
the larvae of
Protophormia
terraenovae
(order Diptera,
family Calliphoridae)
used as live bait
for fishing.
Objective: To
identify the pattern
of major allergens
in P terraenovae
and other
species of
Calliphoridae.
Materials and
Methods:
Extracts of P
terraenovae,
Calliphora vomitoria,
Lucilia sericata
and Lumbricus
terrestris were
characterized using
sodium dodecyl
sulfate
polyacrylamide gel
electrophoresis and
IgE-immunoblotting
techniques in
individual sera from
24 patients with a
positive skin test
result and/or
specific IgE
determination
(enzyme-linked
immunosorbent assay
[ELISA]) to P
terraenovae.
ELISA and
IgE-immunoblotting
inhibition studies
were also performed
to identify
potential
cross-reactive
allergens between
these species.
Results:
IgE-immunoblotting
with P
terraenovae
showed a band of
15.3 kDa recognized
by 15 patients, in
addition to 2
further allergens of
22.8 kDa and 69 kDa.
For C vomitoria,
5 bands of 73, 46,
40, 28, and 14 kDa
were observed. For
L sericata, 2
major allergens of
73 kDa and 14 kDa
were observed. In
the case of L
terrestris, IgE
from 13 patients
recognized 1
allergen of around
15.5 kDa.
IgE-immunoblotting
and ELISA inhibition
revealed the
presence of
cross-reactivity,
mainly between L
terrestris and
P terraenovae.
Conclusions:
P terraenovae
appears to have
species-specific
allergens and
allergens shared
with C vomitoria
and L
sericata.
Striking
immunological
cross-reactivity was
observed between
P terraenovae
and L terrestris.
An allergen of 15-16
kDa could be
involved in this
phenomenon.
Key words:
Protophormia
terraenovae.
Maggot. Allergens.
Allergy. Anglers.
Fish bait. |
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