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Original Article


Comparison of Molecular Multiplex and Singleplex Analysis of IgE to Grass Pollen Allergens in Untreated German Grass Pollen–Allergic Patients


Ahlgrim C1, Gutermuth J2, Önell A3, Borres MP3,4, Schäffner I5, Darsow U6,7, Pfab F6,7, Brockow K6,7, Ring J6, Behrendt H7, Jakob T8, Huss-Marp J5,8

1University Freiburg Medical Center, Department of Exercise Medicine and Sport, University of Freiburg, Freiburg, Germany
2Department of Dermatology, Universitair Ziekenhuis Brussel, Vrije Universiteit Brussel (VUB), Brussels, Belgium
3Thermo Fisher Scientific, Phadia AB, Uppsala, Sweden
4Department of Women’s and Children’s Health, Uppsala University, Uppsala, Sweden
5Thermo Fisher Scientific, Phadia GmbH, Freiburg, Germany
6Department of Dermatology and Allergy, Technische Universität München, Munich, Germany
7ZAUM-Center for Allergy and Environment, Helmholtz Center Munich, Technische Universität München, Munich, Germany
8Allergy Research Group, Department of Dermatology, Medical Center, University of Freiburg, Freiburg, Germany

J Investig Allergol Clin Immunol 2015; Vol. 25(3): 190-195



Background: The ImmunoCAP ISAC 112 platform is the only commercially available molecular allergy IgE multiplex test. Data on the comparison of this rather novel test with the molecular singleplex ImmunoCAP IgE platform are lacking.

Objective: To compare the multiplex ISAC 112 platform and the singleplex ImmunoCAP platform in regard to IgE to grass pollen allergens in untreated grass pollen–allergic patients in Germany.

Methods: Serum samples from 101 adults with grass pollen allergy were analyzed for specific IgE (sIgE) to 8 allergenic molecules from timothy grass pollen and to the 112 allergenic molecules included in the ISAC panel. The results for the multiplex and singleplex tests were subsequently analyzed statistically.

Results: Comparison of sIgE to grass pollen allergens detected by ISAC 112 and the singleplex ImmunoCAP assay revealed the following correlation coefficients: 0.88 (rPhl p 1), 0.96 (rPhl p 2), 0.70 (nPhl p 4), 0.94 (rPhl p 5b), 0.92 (rPhl p 6), 0.85 (rPhl p 11), and 0.78 (rPhl p12).

Conclusion: Molecular testing with ISAC 112 correlates well with the ImmunoCAP platform for respective molecular timothy grass pollen allergens.

Key words: Molecular allergy. Multiplex. ImmunoCAP. ISAC. Component-resolved diagnosis.