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Original Article


5-Lipoxygenase Pathway GenePolymorphisms: Lack of Association With Asthma in a Spanish Population


MJ Torres-Galván,1 JA Cumplido,2 N Buset,1 A Caballero-Hidalgo,1 C Blanco,3 E Hernández,1 T Carrillo2

1Research Unit, Hospital Universitario de Gran Canaria Dr. Negrín, Las Palmas de Gran Canaria, Spain
2Allergology Department, Hospital Universitario de Gran Canaria Dr. Negrín, Las Palmas de Gran Canaria, Spain
3Allergology Department, Hospital Universitario de la Princesa, Madrid, Spain

J Investig Allergol Clin Immunol 2009; Vol. 19(6): 453-458



Background: Cysteinyl-leukotrienes are mediators of inflammatory responses in bronchial asthma. We studied the genes encoding the enzymes involved in their synthesis to identify risk factors for asthma. The promoter polymorphisms LTC4S –444 A/C, ALOX5 –176/–147, and ALOX5AP –169/–146 have been reported to be associated with bronchial asthma.

Methods: We analyzed the effects of LTC4S –444 A/C, ALOX5 –176/–147, and ALOX5AP –169/–146 on asthma susceptibility by means of a case-control study with 193 ethnically matched, unrelated individuals. Participants were classified as severe asthmatics, nonsevere asthmatics, and nonasthmatics, using a combination of 2 techniques: polymerase chain reaction-restricted fragment length polymorphism and multiplex capillary electrophoresis.

Results: No association was found between these polymorphisms and asthma, neither individually nor in combination.

Conclusion: Although the studied polymorphisms have been previously reported to constitute risk factors for the disease, we found no association between LTC4S –444 A/C, ALOX5 –176/–147, and ALOX5AP –169/–146 polymorphisms and bronchial asthma.

Key words: Bronchial asthma. 5-Lipoxygenase pathway. Promoter. Polymorphism. Risk.