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Original Article


Interleukin 13 Receptors as Biochemical Markers in Atopic Patients


YM Hussein,¹ AS Ahmad,² MM Ibrahem,¹ HM Elsherbeny,³ SM Shalaby,¹ AS El-Shal,¹ NA Sabbah¹

1Medical Biochemistry Department, Faculty of Medicine, Zagazig University, Egypt
2Chest Department, Faculty of Medicine, Zagazig University, Egypt
3 Clinical Pathology Department, Faculty of Medicine, Cairo University, Egypt

J Investig Allergol Clin Immunol 2011; Vol. 21(2): 101-107



Background: Interleukin (IL) 13, a type 2 helper T cell (TH2), is an important regulator of inflammatory immune responses. It mediates its action through a receptor complex consisting of IL-13Rα1 and IL-4Rα. IL-13Rα2 binds IL-13 with high affi nity and is thought to act primarily as a decoy receptor, sequestering IL-13 and thus inhibiting its action. Our aim was to clarify the role of these receptors in the diagnosis and follow-up of atopic patients.

Methods: We genotyped the 1398A>G polymorphism in the IL-13Rα1 gene using restriction fragment length polymorphism for causal genetic diversity and measured serum levels of IL-13Rα2 in 105 atopic patients suffering from atopic asthma, atopic dermatitis, and atopic rhinitis (35 each). We compared the results with those of 35 nonatopic control individuals. Total immunoglobulin (Ig) E and serum IL-13Rα2 were measured using enzyme-linked immunosorbent assay, and the eosinophil counts were recorded.

Results: A significant increase in serum IL-13Rα2 levels was recorded in the 3 atopic groups compared with the control group (P<.001), as well as a significant increase in total IgE levels and eosinophil counts. No significant association was found between 1398A>G and atopy other than a suggestive association between this polymorphism and raised total serum IgE levels in all 3 atopic groups (P<.001).

Conclusions: These fi ndings indicate that IL-13Rα2 plays an important role in atopy and that increased levels in different groups highlight its regulatory role in the development of atopic symptoms. The 1398A>G polymorphism might be involved in the production of IgE.

Key words: Atopic patients. Interleukin 13. Asthma.