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J Kayserova,1 K Sismova1
I Zentsova-Jaresova,1 S Katina,2,3
E Vernerova,1 A Polouckova,1 S
Capkova,4 V Malinova,5 I Striz,6
A Sediva1 |
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1Department of Immunology, Charles
University, 2nd Faculty of Medicine, University Hospital
Motol, Prague, Czech Republic
2Department of Applied Mathematics and Statistics,
Faculty of Mathematics, Physics and Informatics,
Comenius University, Bratislava, Slovakia
3Institute of Normal and Pathological Physiology, Slovak
Academy of Sciences, Bratislava, Slovakia
4Paediatric Department of Dermatology, Paediatric
Outpatient Department, University Hospital Motol,
Prague, Czech Republic
5Department of Paediatrics and Adolescent Medicine, 1st
Faculty of Medicine, General University Hospital,
Prague, Czech Republic
6Department of Immunogenetics, Institute for Clinical
and Experimental Medicine, Prague, Czech Republic |
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Abstract |
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Background and
Objective: The
course of atopic
dermatitis (AD) in
childhood is
characterized by
typical changes in
phenotype, including
a shift from skin
involvement to
respiratory allergy
usually around the
third year of age.
We thus designed a
prospective study to
monitor the outcome
of severe AD and to
investigate the
association between
cytokine gene
polymorphisms and
clinical
manifestations.
Methods:
Clinical and
laboratory follow-up
of 94 patients with
severe AD and 103
healthy controls was
performed using
routine methodology.
Allele, genotype,
and haplotype
frequencies of
single nucleotide
polymorphisms of 13
selected
cytokine/receptor
genes were analyzed
using PCR with
sequence-specific
primers.
Results: In
our study, genotypes
of 7
polymorphismsIL-4
-1098G/T and
-590C/T, IL-6
-174C/G and
nt565A/G, and IL-10
-1082A/G,-819C/T,
and -592A/C were
significantly
associated with
atopic AD (P<.05). A
significant
association was also
found for TNF-α AA
and IL-4 GC
haplotypes and AD.
We confirm the
progressive clinical
improvement of AD
together with a
decrease in the
severity index
SCORAD (SCORing
atopic dermatitis)
during childhood
(P<.05). We found
signifi cant
differences between
IL-4Rα +1902 A/G and
positivity of tree
pollenspecific IgE
(P<.05) in the AD
group. Moreover, a
weak association was
also found between
IL-10 -819C/T and
IL-10 -590A/C and
the appearance of
allergic rhinitis
(P<0.1).
Conclusions:
We confirmed a
clinical shift in
allergic phenotype
in the fi rst 3
years of life, and
showed an
association between
IL-4, IL-6, and
IL-10 polymorphisms
and AD. Our data
indicate that IL-4α
and IL-10
polymorphisms may be
considered
predictive factors
of respiratory
allergy in children
with AD.
Key words:
Allergic rhinitis.
Allergy. Atopic
dermatitis. Single
nucleotide
polymorphism.
Cytokines.
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