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Original Article


Is the ISAC 112 Microarray Useful in the Diagnosis of Pollinosis in Spain?


García BE1,2, Martínez-Aranguren R2,3, Bernard Alonso A3, Gamboa P4, Feo Brito F5, Bartra J6, Blanca-López N7, Gómez F8, Alvarado MI9, Fernández J10, Alonso MD11, Gonzalez-Mancebo E12, Moya C13, Parra A14, Terrados S15, Sola L1, Goikoetxea MJ2,3, Sanz ML2,3

1Allergology Service, Complejo Hospitalario de Navarra, Pamplona, Spain
2IdiSNA, Navarra Institute for Health Research, Spain
3Allergology and Clinical Immunology Department, Clínica Universidad de Navarra, Pamplona, Spain
4Allergology Service, Hospital de Basurto, Bilbao, Spain
5Allergology Service, Hospital General Universitario de Ciudad Real, Ciudad Real, Spain
6Unitat d’Alèrgia Servei Pneumologia i Alèrgia Respiratoria Universitat de Barcelona, Institut d’Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), Centro de Investigaciones Biomédicas en Red de Enfermedades Respiratorias (CIBERES), Barcelona, Spain
7Allergology Service, Hospital Infanta Leonor, Madrid, Spain
8Allergology Service, IBIMA, Hospital Regional Universitario de Malaga, UMA, Malaga, Spain
9Allergology Service, Hospital Virgen del Puerto, Plasencia, Cáceres, Spain
10Allergology Service, Hospital General Universitario de Alicante, Alicante, Spain
11Allergology Service, Hospital de Alcorcón, Alcorcón, Spain
12Allergology Service, Hospital de Fuenlabrada, Madrid, Spain
13Allergology Service, Complejo Hospitalario Torrecárdenas, Almería, Spain
14Allergology Service, Hospital de A Coruña, A Coruña, Spain
15Allergology Service, Hospital Universitario Ramon y Cajal, IRYCIS, Madrid, Spain

J Investig Allergol Clin Immunol 2016; Vol. 26(2): 92-99
doi: 10.18176/jiaci.0052



Background: Multiple sensitization is frequent among pollen-allergic patients. The goal of this study was to determine the diagnostic accuracy of the ImmunoCAP ISAC 112 (ISAC112) microarray in allergy to pollen from several taxa and its clinical utility in a Spanish population.

Methods: Specific IgE was determined in 390 pollen-allergic patients using the ISAC112 microarray. Diagnostic accuracy (sensitivity, specificity, predictive values, and area under the ROC curve) was calculated for the diagnosis of allergy to pollen from grass (n=49), cypress (n=75), olive tree (n=33), plane tree (n=63), and pellitory of the wall (n=17) and compared with that of the singleplex ImmunoCAP immunoassay.

Results: The sensitivity of the ISAC112 microarray ranged from 68.2% for allergy to plane tree pollen to 93.9% for allergy to grass pollen. The specificity was >90%. The AUC for the diagnosis of allergy to plane tree pollen was 0.798, whereas the AUC for the remaining cases was ≥0.876. The accuracy of ISAC112 was higher than that of ImmunoCAP for plane tree pollen and similar for the remaining pollens. The frequency of sensitization to most species-specific allergenic components and profilins varied between the different geographical regions studied. A total of 73% of pollen-allergic patients were sensitized to species-specific components of more than 1 pollen type.

Conclusions: The ISAC112 microarray is an accurate tool for the diagnosis of allergy to pollen from grass, cypress, olive tree, plane tree, and pellitory of the wall. The features of the ISAC112 microarray are similar or superior (in the case of plane tree pollen) to those of ImmunoCAP. This microarray is particularly useful for the etiologic diagnosis of pollinosis in patients sensitized to multiple pollen species whose pollination periods overlap.

Key words: Microarray. Pollen allergy. Molecular diagnosis. Accuracy.