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Api m 6 and Api m 10 as Major Allergens in Patients With Honeybee Venom Allergy

Vega-Castro A1, Rodríguez-Gil D2, Martínez-Gomariz M2, Gallego R2, Peña MI1, Palacios R2

1Hospital Universitario de Guadalajara, Guadalajara, Spain; Spanish Thematic Network and Co-operative Research Centre ARADyAL, Center RD16/0006/0023
2Diater Laboratorios S.A., Leganés, Madrid, Spain

J Investig Allergol Clin Immunol 2022; Vol 32(2) : 116-123
doi: 10.18176/jiaci.0639

Background: Component-resolved diagnosis plays a key role in the diagnosis and treatment of honeybee venom allergy (HVA). Our aim was to study whether any of the allergens not included in the usual diagnostic platforms are relevant in our population.
Patients and Methods: The allergenic sensitization profile of Spanish patients who experienced a systemic reaction after a honeybee sting and were diagnosed with HVA was studied by immunoblotting based on raw autochthonous Apis mellifera venom characterized using SDS-PAGE and mass spectrometry and a commercial assay (ImmunoCAP).
Results: Allergens in the International Union of Immunological Societies database were detected in the raw A mellifera venom extract used, except Api m 12. Sera from 51 patients with a median (IQR) age of 46.2 years (35.6-54.6) were analyzed. ImmunoCAP revealed Api m 1 and Api m 10 to be major allergens (88.2% and 74.5%, respectively). Moreover, Api m 6 (85.4%) was detected by immunoblotting.
Conclusion: Api m 1, Api m 6, and Api m 10 are major A mellifera venom allergens in our population.

Key words: Bee venom allergy, Component-resolved diagnostics, Venom sensitization profile, Api m 6, Api m 10

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